Preparation of plasma-membrane subfractions from isolated rat hepatocytes
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چکیده
منابع مشابه
Preparation of Plasma Membrane from Isolated Neurons
A bulk fraction enriched with respect to neuronal cell bodies was used as starting material for the isolation of neuronal plasma membrane The cells were gently homogenized in isotonic sucrose and a crude membrane containing fraction sedimented at 3000 g. Subsequently, the membrane fraction was purified on a discontinuous sucrose density gradient between 35% and 25 5% sucrose (w/w). Enzymatic an...
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Phospholipase C treatment of vesicular subfractions of plasma membranes derived from the three functional domains of rat liver indicated that there is an asymmetric distribution of phospholipids across the bilayer of these membranes. The bile-canalicular and sinusoidal membranes were similar and different from the contiguous membrane.
متن کاملPreparation of isolated rat liver hepatocytes.
The bulk volume (about 85%) of the mammalian liver parenchyma is contributed by the hepatocytes, whereas at least four other types of cells constitute the remainder (1). Procedures for the isolation of all five cell types have been described, although not from the same liver. However, the isolation of hepatocytes has clearly been the most widely used preparation and has proved extremely valuabl...
متن کاملPreparation of isolated periportal or perivenous hepatocytes from rat liver.
There are a number of functional, metabolic differences between periportal and perivenous hepatocytes in the mammalian liver resulting from zonal differences in the activity of several enzymes, and possibly from morphological differences as well (1). Examples of key enzymes where the periportal/perivenous activity ratio is >1 are glucose-σ-phosphatase (2), phosphoenolpyruvate carboxykinase (3),...
متن کاملUptake of iron from transferrin by isolated rat hepatocytes. A redox-mediated plasma membrane process?
The uptake of iron from transferrin by isolated rat hepatocytes varies in parallel with plasma membrane NADH:ferricyanide oxidoreductase activity, is inhibited by ferricyanide, ferric, and ferrous iron chelators, divalent transition metal cations, and depends on calcium ions. Iron uptake does not depend on endosomal acidification or endocytosis of transferrin. The results are compatible with a ...
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ژورنال
عنوان ژورنال: Biochemical Journal
سال: 1977
ISSN: 0306-3283
DOI: 10.1042/bj1640415